Flow Cytometry

Overview

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

This information is general and should not be treated as medical advice. Always speak directly with a qualified healthcare professional before making decisions about treatment, diagnosis, procedures, medicines or care.

Quick facts about Flow Cytometry

Topic type

Diagnostic Test

Category

Diagnostics

MeSH classification

E01.370.225.500.363.342|E01.370.225.500.386.350|E05.196.712.516.600.240.350|E05.200.500.363.342|E05.200.500.386.350|E05.242.363.342|E05.242.386.350

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