Freeze Etching

Overview

A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.

This information is general and should not be treated as medical advice. Always speak directly with a qualified healthcare professional before making decisions about treatment, diagnosis, procedures, medicines or care.

Quick facts about Freeze Etching

Topic type

Diagnostic Test

Category

Diagnostics

MeSH classification

E01.370.225.500.620.620.260.400|E01.370.225.750.600.620.260.400|E05.200.500.620.620.260.400|E05.200.750.600.620.260.400

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